Forskolin-Induced Swelling Assay
The Forskolin-Induced Swelling (FIS) assay is an imaging-based assay that measures organoid swelling as a readout for Cystic Fibrosis Transmembrane conductance Regulator (CFTR) protein function before and after treatment to provide indications on a new drug efficacy and patient response. The FIS assay is a valuable tool in Cystic Fibrosis (CF) drug development and patient stratification and has already shown its potential for clinical patient representation and personalized medicine.
Epithelial barrier function assay
The Transepithelial Electrical Resistance (TEER) assay is designed to analyze tight junction dynamics and determine barrier function integrity of an intestinal epithelium. Organoids are established as a ‘2D’ monolayer in Transwell®.
Immune-mediated tumor organoid killing assay
We have developed an organoid and immune cell co-culture platform that uses native or engineered immune cells admixed with normal or tumor organoids to evaluate antigen specific recognition/killing of tumor organoids compared to healthy controls. These co-cultures can also be applied to testing new immuno modulatory compounds.
