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Immune-mediated tumor organoid killing assay

Our organoid and immune cell co-culture platform can be used to evaluate new immunomodulatory compounds such as bi-specifics, checkpoint inhibitors, or CAR-T cells. Our standardized assay format allows to measure reactivity of high-dose-IL-2 expanded, unbiased/polyclonal Tumor-Infiltrating Lymphocytes (TIL) against paired cancer patient-derived HUB Organoids™. TILs reactivity is measured by quantifying the level of induced tumor organoid cell death. If paired TIL are not available, non-paired T cell clones and HLA-matched peptide pulsed organoids can be exploited.

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Quick facts

  • Assay layout: 96-well plate format
  • Read out: organoid cell death and/or IFNγ production
  • Application area: immuno-oncology

Assay validated on the following HUB Organoid™ models

  • Colon
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Additional services

  • Genomics services
  • Imaging
  • Cytokine analysis by ELISA 
  • Flow cytometry for phenotyping and to assess TIL viability

On request, we can set up specific assays that are not listed and which we have not yet developed.

Want to know more?

Please contact us at +31 (0)88 12 36 300 or email us info@huborganoids.nl or drop us a message via the button below.

contact us
  • Yalelaan 62
  • 3584 CM Utrecht
  • The Netherlands