Epithelial barrier function assays
We have successfully grown HUB Organoids® as 2D monolayers on Transwells to analyze tight-junction dynamics and determine barrier function integrity of epithelia in response to various external stimuli, like a drug or chemical compound. Assays results are used to determine your drug EC50/IC50 and can be presented as dose response curves and/or IC/EC values.
- Barrier function/integrity is evaluated by measuring the trans epithelial electrical resistance (TEER)
- Transport function is assesses by measuring a fluorescent readout such as a PGP probe, across the organoid membrane
- Florescent markers such as Lucifer Yellow or Dextran-FITC are measured to evaluate paracellular permeability
- Cell viability is determined by quantifying the cellular ATP content
