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Drug screening with HUB Organoids™

HUB Organoids are amenable to a wide range of standard in vitro assays typically used in drug screens, such as measuring organoid cell viability and proliferation following treatment with your test agent. Additionally, we have developed organ and disease specific assays including complex high-content imaging or -omics to study drug response, which are available as a service and through licensing. Last but not least, we have validated different format assays on organoid which can be run in the native ‘3D’ cystic or budding shapes, as well as ‘2D’ epithelia to study how your compound affects barrier function integrity, tight junction dinamics, permeability, transport, and interaction with the microbiome.

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Cell viability assay

We offer robust and innovative assays for determining cell viability, proliferation, and cytotoxicity which have been developed in a 3D format and can be multiplexed with imaging. Using 384-well plates, up to 12,000 data points can be measured in one single assay to test the efficacy and toxicity of drugs.

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Forskolin-Induced Swelling Assay

The Forskolin-Induced Swelling (FIS) assay is an imaging-based assay that measures organoid swelling as a readout for Cystic Fibrosis Transmembrane conductance Regulator (CFTR) protein function before and after treatment to provide indications on a new drug efficacy and patient response. The FIS assay is a valuable tool in Cystic Fibrosis (CF) drug development and patient stratification and has already shown its potential for clinical patient representation and personalized medicine.

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Epithelial barrier function assay

The Transepithelial Electrical Resistance (TEER) assay is designed to analyze tight junction dynamics and determine barrier function integrity of an intestinal epithelium. Organoids are established as a ‘2D’ monolayer in Transwell®.

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Immune-mediated tumor organoid killing assay

We have developed an organoid and immune cell co-culture platform that uses native or engineered immune cells admixed with normal or tumor organoids to evaluate antigen specific recognition/killing of tumor organoids compared to healthy controls. These co-cultures can also be applied to testing new immuno modulatory compounds.

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