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Cell viability assay

This standard in vitro assay which uses ATP readout as a measure of cell viability can be applied to any of our HUB Organoid™ models. Cell viability data are then used to determine EC50/IC50 and GR50 (or other parameters such as IC90). Results are presented as dose response curves and/or IC/EC/GR values.

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Quick facts

  • Assay layout: 96- and 384-well plate format
  • Read out: measurement of cellular ATP levels
  • Application area: oncology, toxicology, IBD, COPD

Assay validated on the following HUB Organoid™ models

  • Human: bladder, breast, colon, head and neck, kidney*, liver*, lung, ovarian, pancreas, small intestine, stomach
  • Rat: small intestine*
  • Dog: colon*, small intestine*
  • Guinea pig: colon*, small intestine*
  • Mini pig: colon*, small intestine*

*only normal/healthy organoids

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Additional services

  • Genomics
  • Immunohistochemistry
  • Gene and protein expression analysis via RT-PCR or RNAseq, Western Blot, FACS
  • Imaging for end point measurements
  • Cytokine secretion analysis by ELISA

Custom made assay development and set up can be carried upon request.

Want to know more?

Please contact us at +31 (0)88 12 36 300 or email us info@huborganoids.nl or drop us a message via the button below.

contact us
  • Yalelaan 62
  • 3584 CM Utrecht
  • The Netherlands
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